[Comparative study on 1D [VP1] region of foot and mouth disease virus [type A strain] among different isolates: Khorasan Razavi isolate and other Iranian and neighboring countries isolates]

Foot-and-mouth disease [FMD] is one of the most important virus disease in farm animals. Types O, A and Asial FMD virus have been endemic in Iran. In this study, samples from suspected livestock were analyzed by RT-PCR experiment. The number of 702 nucleotides determined at 1D- 2B region of type A strain isolated from Khorasan Razavi province sequenced and compared with that of other reported isolates type A from Iran and neighboring countries. The results show that field isolated type A has about 89% similarity with other reported isolates type A from Iran and neighboring countries. Furthermore, this virus shows the most similarity with A/IRN/1/87[Samuel. Phylogenitic analysis revealed that virus was closely related to A22-Iraq/99 and A/IRN/iso/105 that rest in the same lineage. The data showed high similarity between type A viruses involved in the Khorasan Razavi province and A/IRN/87v [vaccine strain]; so that it can be concluded that the vaccine can produce prophylactic antibody against this virus

Identification of the foot and mouth disease foci from susceptible foci in Khorasan Razavi province

This study was conducted in Khorasan-Razavi during 23 months [since February 2004 to December 2005]. The samples [83 samples] of suspected farms were collected from tongue and mouth epithelium and tested by indirect sandwich ELISA and RT-PCR. During the study, twelve foci during the year 2004 and 23 foci during the year 2005[up to December] were approved. Maximum distribution of FMD foci was observed in spring [63%]. Pearson's correlation coefficient and Chi- square tests were carried out for data analysis. Attention was paid to frequent traffic of farm in springs since there is an increase of the disease in this season. There was no correlation between the sheep density and the disease must be account in cattle. However, with respect to considerable sheep population in Iran and its role in persistence of the virus, the vaccination of sheep seems to be essential for the control of FMD